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HES5 Antibody (YA1449, Rabbit)

HY-P81704
MedChem Express
TargetHES5
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    HES5 Antibody (YA1449, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID388585
  • Target name
    HES5
  • Target description
    hes family bHLH transcription factor 5
  • Target synonyms
    bHLHb38, transcription factor HES-5, class B basic helix-loop-helix protein 38, hairy and enhancer of split 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    HES5 Antibody (YA1449) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HES5.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HES5 using anti-HES5 antibody (A05384-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HES5 antigen affinity purified polyclonal antibody (Catalog # A05384-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HES5 at approximately 18 kDa. The expected band size for HES5 is at 18 kDa.
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