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Hex Antibody (YA1902, Rabbit)

HY-P82157
MedChem Express
TargetHHEX
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Hex Antibody (YA1902, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3087
  • Target name
    HHEX
  • Target description
    hematopoietically expressed homeobox
  • Target synonyms
    hematopoietically-expressed homeobox protein HHEX; HEX; HMPH; homeobox protein HEX; homeobox protein PRH; homeobox, hematopoietically expressed; HOX11L-PEN; PRH; PRHX; proline-rich homeodomain-containing transcription factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Hex Antibody (YA1902) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1902), targeting Hex, with a predicted molecular weight of 30 kDa (observed band size: 30 kDa). Hex Antibody (YA1902) can be used for WB, IP experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Hex using anti-Hex antibody (PB9631). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hex antigen affinity purified polyclonal antibody (Catalog # PB9631) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hex at approximately 40 kDa. The expected band size for Hex is at 40 kDa.
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