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ProductsBack/HHEX / HEX Antibody (aa146-180)

HHEX / HEX Antibody (aa146-180)

LS-C407843
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetHHEX
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HHEX / HEX Antibody (aa146-180)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3087
  • Target name
    HHEX
  • Target description
    hematopoietically expressed homeobox
  • Target synonyms
    hematopoietically-expressed homeobox protein HHEX; HEX; HMPH; homeobox protein HEX; homeobox protein PRH; homeobox, hematopoietically expressed; HOX11L-PEN; PRH; PRHX; proline-rich homeodomain-containing transcription factor
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hex using anti-Hex antibody (PB9631). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hex antigen affinity purified polyclonal antibody (Catalog # PB9631) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hex at approximately 40 kDa. The expected band size for Hex is at 40 kDa.
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