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HFip1 / FIP1L1 Antibody

LS-C346244
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetFIP1L1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HFip1 / FIP1L1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 40kDa/58kDa/65kDa/66kDa, while the observed MW by Western blot was 75kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 40kDa/58kDa/65kDa/66kDa, while the observed MW by Western blot was 75kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID81608
  • Target name
    FIP1L1
  • Target description
    factor interacting with PAPOLA and CPSF1
  • Target synonyms
    factor interacting with PAP; FIP1; FIP1 like 1; FIP1L1 cleavage and polyadenylation specific factor subunit; FIP1-like 1 protein; hFip1; pre-mRNA 3'-end-processing factor FIP1; rearranged in hypereosinophilia; Rhe
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FIP1L1 antigen affinity purified polyclonal antibody (Catalog # A02452-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FIP1L1 at approximately 72 kDa. The expected band size for FIP1L1 is at 67 kDa.
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