HIF1 alpha Antibody (OASE00130)

Aviva Systems Biology

HIF1 alpha Antibody (OASE00130)

23

ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

Monoclonal

ESEE122

1 mg/ml

Unconjugated

Hif1a

hypoxia inducible factor 1, alpha subunit

HIF-1-alpha, HIF1-alpha, HIF1alpha, MOP1, bHLHe78, hypoxia-inducible factor 1-alpha, ARNT-interacting protein

Mouse

IgG1

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed Hif-1alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, Hif-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit Hif-1alpha degradation and lead to its stabilization. In addition, Hif-1alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).

-20°C

12352203