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WB analysis of various samples using GTX04988 HIF3 alpha antibody. Lane 1 : Rat lung tissue lysate Lane 2 : Rat skeletal muscle tissue lysate Lane 3 : Mouse lung tissue lysate Lane 4 : NIH3T3 whole cell lysate Loading : 50μg Dilution : 0.5 μg/mL
WB analysis of various samples using GTX04988 HIF3 alpha antibody. Lane 1 : Rat lung tissue lysate Lane 2 : Rat skeletal muscle tissue lysate Lane 3 : Mouse lung tissue lysate Lane 4 : NIH3T3 whole cell lysate Loading : 50μg Dilution : 0.5 μg/mL
WB analysis of various samples using GTX04988 HIF3 alpha antibody. Lane 1 : Rat lung tissue lysate Lane 2 : Rat skeletal muscle tissue lysate Lane 3 : Mouse lung tissue lysate Lane 4 : NIH3T3 whole cell lysate Loading : 50μg Dilution : 0.5 μg/mL

HIF3 alpha antibody

GTX04988
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHIF3A
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Overview

  • Supplier
    GeneTex
  • Product Name
    HIF3 alpha antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.25-0.5 microg/ml. FACS: 1-3 microg/1x106 cells. ELISA: 0.1-0.5 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64344
  • Target name
    HIF3A
  • Target description
    hypoxia inducible factor 3 subunit alpha
  • Target synonyms
    HIF-3A, HIF3-alpha-1, IPAS, MOP7, PASD7, bHLHe17, hypoxia-inducible factor 3-alpha, PAS domain-containing protein 7, basic-helix-loop-helix-PAS protein MOP7, class E basic helix-loop-helix protein 17, hypoxia inducible factor 3 alpha subunit, inhibitory PAS domain protein, member of PAS protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2N7
  • Protein Name
    Hypoxia-inducible factor 3-alpha
  • Scientific Description
    The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
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