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Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody.
Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody.
Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody.

Histone H2AX Antibody

CSB-PA833019
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetH2AX
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Overview

  • Supplier
    Cusabio
  • Product Name
    Histone H2AX Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3014
  • Target name
    H2AX
  • Target description
    H2A.X variant histone
  • Target synonyms
    H2A histone family member X; H2A.X; H2A/X; H2AFX; H2AX histone; histone H2A.x; histone H2AX
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16104
  • Protein Name
    Histone H2AX
  • Scientific Description
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. The MGC Project Team; Genome Res. 14:2121-2127(2004). Rogakou E.P., J. Biol. Chem. 273:5858-5868(1998). Rogakou E.P., J. Biol. Chem. 275:9390-9395(2000).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of H2AFX using anti-H2AFX antibody (A00241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFX antigen affinity purified polyclonal antibody (Catalog # A00241-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFX at approximately 15 kDa. The expected band size for H2AFX is at 15 kDa.
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