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ProductsBack/Histone H2A.Z antibody - ChIP grade
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60812 Histone H2A.Z antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 microg per ChIP experiment was analysed. IgG (1 microg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes c-fos and EIF2S3, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60812 Histone H2A.Z antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 microg per ChIP experiment was analysed. IgG (1 microg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes c-fos and EIF2S3, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60812 Histone H2A.Z antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 microg per ChIP experiment was analysed. IgG (1 microg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes c-fos and EIF2S3, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Histone H2A.Z antibody - ChIP grade

GTX60812
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
ReactivityHuman
TargetH2AZ1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Histone H2A.Z antibody - ChIP grade
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1,000. ICC/IF: 1:500. ELISA: 1:5,000. ChIP assay: 0.5-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.55 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3015
  • Target name
    H2AZ1
  • Target description
    H2A.Z variant histone 1
  • Target synonyms
    H2A histone family member Z; H2A.z; H2A.Z-1; H2A/z; H2AFZ; H2AZ; H2AZ histone; histone H2A.Z
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP0C0S5
  • Protein Name
    Histone H2A.Z
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203