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ProductsBack/Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade
ChIP analysis of sheared chromatin from 1.6x10? U2OS cells using GTX60356 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade. A titration of the antibody consisting of 2, 5, 10 and 15 microl per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. Quantitative PCR was performed using primers for the promoter of the housekeeping gene GAPDH and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 1.6x10? U2OS cells using GTX60356 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade. A titration of the antibody consisting of 2, 5, 10 and 15 microl per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. Quantitative PCR was performed using primers for the promoter of the housekeeping gene GAPDH and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 1.6x10? U2OS cells using GTX60356 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade. A titration of the antibody consisting of 2, 5, 10 and 15 microl per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. Quantitative PCR was performed using primers for the promoter of the housekeeping gene GAPDH and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade

GTX60356
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
ReactivityHuman
TargetH3C1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1,000. ICC/IF: 1:200. Dot: 1:200,000. ELISA: 1:2,000 - 1:3,000. ChIP assay: 10 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8350
  • Target name
    H3C1
  • Target description
    H3 clustered histone 1
  • Target synonyms
    H3 histone family, member A; H3/A; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; HIST1H3A; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; histone H3.1; histone H3/a
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203