ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60345 Histone H4 antibody - ChIP grade. A titration consisting of 0.5, 1, 2 and 5 microg of antibody per ChIP experiment was analyzed. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and c-fos genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Histone H4 antibody - ChIP grade
GTX60345
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetH4C1
Overview
- SupplierGeneTex
- Product NameHistone H4 antibody - ChIP grade
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.3 mg/ml
- ConjugateUnconjugated
- Gene ID8359
- Target nameH4C1
- Target descriptionH4 clustered histone 1
- Target synonymsH4 histone family, member A; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FA; HIST1H4A; histone 1, H4a; histone cluster 1 H4 family member a; histone cluster 1, H4a; histone H4
- HostRabbit
- IsotypeIgG
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203