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Western Blot Positive WB detected in: U87 whole cell lysate All lanes: HK2 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 103 kDa Observed band size: 103 kDa
Western Blot Positive WB detected in: U87 whole cell lysate All lanes: HK2 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 103 kDa Observed band size: 103 kDa
Western Blot Positive WB detected in: U87 whole cell lysate All lanes: HK2 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 103 kDa Observed band size: 103 kDa

HK2 Antibody

CSB-PA010469LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetHK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    HK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3099
  • Target name
    HK2
  • Target description
    hexokinase 2
  • Target synonyms
    hexokinase type II; hexokinase-2; hexokinase-2, muscle; hexokinase-B; HKII; HXK2; muscle form hexokinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52789
  • Protein Name
    Hexokinase-2
  • Scientific Description
    cytosol, membrane, mitochondrial outer membrane, fructokinase activity, glucokinase activity, hexokinase activity, mannokinase activity, apoptotic mitochondrial changes, canonical glycolysis, cellular glucose homeostasis
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HK2 using anti-HK2 antibody (A01389). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HK2 antigen affinity purified polyclonal antibody (Catalog # A01389) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HK2 at approximately 102 kDa. The expected band size for HK2 is at 102 kDa.
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