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Western blot All lanes: HKDC1 antibody at 4microg/ml + U87 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 103, 91, 83 kDa Observed band size: 103 kDa
Western blot All lanes: HKDC1 antibody at 4microg/ml + U87 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 103, 91, 83 kDa Observed band size: 103 kDa
Western blot All lanes: HKDC1 antibody at 4microg/ml + U87 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 103, 91, 83 kDa Observed band size: 103 kDa

HKDC1 Antibody

CSB-PA647983LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHKDC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HKDC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80201
  • Target name
    HKDC1
  • Target description
    hexokinase domain containing 1
  • Target synonyms
    RP92, hexokinase HKDC1, hexokinase domain-containing protein 1, putative hexokinase HKDC1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ2TB90
  • Protein Name
    Hexokinase HKDC1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HKDC1 using anti-HKDC1 antibody (PB9375). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HKDC1 antigen affinity purified polyclonal antibody (Catalog # PB9375) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HKDC1 at approximately 103 kDa. The expected band size for HKDC1 is at 103 kDa.
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