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HKDC1 Antibody (HRP)

LS-C395133
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetHKDC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HKDC1 Antibody (HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID80201
  • Target name
    HKDC1
  • Target description
    hexokinase domain containing 1
  • Target synonyms
    RP92, hexokinase HKDC1, hexokinase domain-containing protein 1, putative hexokinase HKDC1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HKDC1 using anti-HKDC1 antibody (PB9375). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HKDC1 antigen affinity purified polyclonal antibody (Catalog # PB9375) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HKDC1 at approximately 103 kDa. The expected band size for HKDC1 is at 103 kDa.
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