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HLA DPB1 Antibody (YA3273)

HY-P83528
MedChem Express
TargetHLA-DPB1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    HLA DPB1 Antibody (YA3273)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3115
  • Target name
    HLA-DPB1
  • Target description
    major histocompatibility complex, class II, DP beta 1
  • Target synonyms
    DPB1; HLA class II histocompatibility antigen, DP beta 1 chain; HLA class II histocompatibility antigen, DP(W4) beta chain; HLA-DP; HLA-DP histocompatibility type, beta-1 subunit; HLA-DP1B; HLA-DPB; MHC class II antigen DPB1; MHC class II HLA-DP-beta-1; MHC HLA DPB1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    HLA DPB1 Antibody (YA3273) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3273), targeting HLA DPB1, with a predicted molecular weight of 29 kDa (observed band size: 29 kDa). HLA DPB1 Antibody (YA3273) can be used for WB, IHC-P, ICC/IF, IP experiment in human background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HLA-DPB1 using anti-HLA-DPB1 antibody (A00487). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DPB1 antigen affinity purified polyclonal antibody (Catalog # A00487) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DPB1 at approximately 29 kDa. The expected band size for HLA-DPB1 is at 29 kDa.
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