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HLA-DPB1 Polyclonal Antibody

CAC14140
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHLA-DPB1
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Overview

  • Supplier
    Biomatik
  • Product Name
    HLA-DPB1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3115
  • Target name
    HLA-DPB1
  • Target description
    major histocompatibility complex, class II, DP beta 1
  • Target synonyms
    DPB1, HLA-DP, HLA-DP1B, HLA-DPB, HLA class II histocompatibility antigen, DP beta 1 chain, HLA class II histocompatibility antigen, DP(W4) beta chain, HLA-DP histocompatibility type, beta-1 subunit, MHC HLA DPB1, MHC class II HLA-DP-beta-1, MHC class II antigen DPB1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04440
  • Protein Name
    HLA class II histocompatibility antigen, DP beta 1 chain
  • Scientific Description
    The HLA-DPB1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HLA-DPB1 using anti-HLA-DPB1 antibody (A00487). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DPB1 antigen affinity purified polyclonal antibody (Catalog # A00487) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DPB1 at approximately 29 kDa. The expected band size for HLA-DPB1 is at 29 kDa.
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