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Hla-Dqb1 Polyclonal Antibody

CAC11907
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetHLA-DQB1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Hla-Dqb1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:200-1:3000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3119
  • Target name
    HLA-DQB1
  • Target description
    major histocompatibility complex, class II, DQ beta 1
  • Target synonyms
    CELIAC1, HLA-DQB, IDDM1, HLA class II histocompatibility antigen, DQ beta 1 chain, MHC class II DQ beta chain, MHC class II HLA-DQ beta glycoprotein, MHC class II antigen DQB1, MHC class II antigen HLA-DQ-beta-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01920
  • Protein Name
    HLA class II histocompatibility antigen, DQ beta 1 chain
  • Scientific Description
    The Hla-Dqb1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HLA-DQB1 using anti-HLA-DQB1 antibody (A00106-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DQB1 antigen affinity purified polyclonal antibody (Catalog # A00106-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DQB1 at approximately 30KD. The expected band size for HLA-DQB1 is at 30KD.
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