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Western Blot analysis of PC12 cells using HLA-DQB1/2 Polyclonal Antibody.
Western Blot analysis of PC12 cells using HLA-DQB1/2 Polyclonal Antibody.
Western Blot analysis of PC12 cells using HLA-DQB1/2 Polyclonal Antibody.

HLA-DQB1/HLA-DQB2 Antibody

CSB-PA005915
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetHLA-DQB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HLA-DQB1/HLA-DQB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3119
  • Target name
    HLA-DQB1
  • Target description
    major histocompatibility complex, class II, DQ beta 1
  • Target synonyms
    CELIAC1, HLA-DQB, IDDM1, HLA class II histocompatibility antigen, DQ beta 1 chain, MHC class II DQ beta chain, MHC class II HLA-DQ beta glycoprotein, MHC class II antigen DQB1, MHC class II antigen HLA-DQ-beta-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01920
  • Protein Name
    HLA class II histocompatibility antigen, DQ beta 1 chain
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HLA-DQB1 using anti-HLA-DQB1 antibody (A00106-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DQB1 antigen affinity purified polyclonal antibody (Catalog # A00106-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DQB1 at approximately 30KD. The expected band size for HLA-DQB1 is at 30KD.
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