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HMG1 / HMGB1 Antibody (aa91-120, PE)

LS-C259786
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetHMGB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HMG1 / HMGB1 Antibody (aa91-120, PE)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    RPE
  • Estimated Purity
    ...
  • Gene ID3146
  • Target name
    HMGB1
  • Target description
    high mobility group box 1
  • Target synonyms
    Amphoterin; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; HMG-1; HMG3; SBP-1; Sulfoglucuronyl carbohydrate binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HMGB1 using anti-HMGB1 antibody (A00066-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGB1 antigen affinity purified polyclonal antibody (Catalog # A00066-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
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