
HMGB1 antibody detects HMGB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HMGB1 stained by HMGB1 antibody (GTX101277) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HMGB1 antibody
GTX101277
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Rat, Zebra Fish
TargetHMGB1
Overview
- SupplierGeneTex
- Product NameHMGB1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.43 mg/ml
- ConjugateUnconjugated
- Gene ID3146
- Target nameHMGB1
- Target descriptionhigh mobility group box 1
- Target synonymsHMG-1, HMG1, HMG3, SBP-1, high mobility group protein B1, Amphoterin, Sulfoglucuronyl carbohydrate binding protein, high-mobility group (nonhistone chromosomal) protein 1
- HostRabbit
- IsotypeIgG
- Protein IDP09429
- Protein NameHigh mobility group protein B1
- Scientific DescriptionDNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
- ReactivityHuman, Mouse, Porcine, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161














![Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2059] (GTX637963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637963/GTX637963_T-44886_20221209_WB_M_R_22121123_430.webp)
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [HL2060] (GTX637964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637964/GTX637964_T-44886_20230116_WB_23011618_157.webp)
![IHC-P analysis of human placenta (amnion and chorion) tissue using GTX639941 HMGB1 antibody [HMV317] HistoMAX?. Strong nuclear HMGB1 staining of chorion cells and moderate nuclear HMGB1 staining of amnion cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639941/GTX639941_20240514_IHC-P_24051319_526.webp)