Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] (GTX629400) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
![HMGB1 antibody [GT412] detects HMGB1 protein at nucleus in human ovarian cancer by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. HMGB1 antibody [GT412] (GTX629400) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX629400/GTX629400_41323_20160616_IHC-P_w_23061202_147.webp "HMGB1 antibody [GT412] detects HMGB1 protein at nucleus in human ovarian cancer by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. HMGB1 antibody [GT412] (GTX629400) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![HMGB1 antibody [GT412] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody [GT412] (GTX629400) diluted at 1:500. Red: GFAP, a glia cell marker, stained by GFAP antibody (GTX27260) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX629400/GTX629400_41323_20170727_IFA_R_w_23061202_419.webp "HMGB1 antibody [GT412] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody [GT412] (GTX629400) diluted at 1:500. Red: GFAP, a glia cell marker, stained by GFAP antibody (GTX27260) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![HMGB1 antibody [GT412] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] (GTX629400) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629400/GTX629400_41323_20151015_WB_Merged_w_23061202_526.webp "HMGB1 antibody [GT412] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] (GTX629400) diluted at 1:1000.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] (GTX629400) diluted at 1:3000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629400/GTX629400_41323_20160602_WB_shRNA_watermark_w_23061202_884.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] (GTX629400) diluted at 1:3000.")
![HMGB1 antibody [GT412] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody [GT412] (GTX629400) diluted at 1:500. Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX629400/GTX629400_41323_20170705_IFA_R_w_23061202_256.webp "HMGB1 antibody [GT412] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody [GT412] (GTX629400) diluted at 1:500. Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![HMGB1 antibody [GT412] detects HMGB1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE HMGB1 antibody [GT412] (GTX629400) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX629400/GTX629400_41323_WB_w_23061202_147.webp "HMGB1 antibody [GT412] detects HMGB1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE HMGB1 antibody [GT412] (GTX629400) dilution: 1:1000")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] (GTX629400) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
HMGB1 antibody [GT412]
GTX629400
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHMGB1
Overview
- SupplierGeneTex
- Product NameHMGB1 antibody [GT412]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT412
- Concentration0.79 mg/ml
- ConjugateUnconjugated
- Gene ID3146
- Target nameHMGB1
- Target descriptionhigh mobility group box 1
- Target synonymsHMG-1, HMG1, HMG3, SBP-1, high mobility group protein B1, Amphoterin, Sulfoglucuronyl carbohydrate binding protein, high-mobility group (nonhistone chromosomal) protein 1
- HostMouse
- IsotypeIgG2a
- Protein IDP09429
- Protein NameHigh mobility group protein B1
- Scientific DescriptionDNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Sheu ML, Pan LY, Yang CN, et al. Neuronal Death Caused by HMGB1-Evoked via Inflammasomes from Thrombin-Activated Microglia Cells. Int J Mol Sci. 2023,24(16). doi: 10.3390/ijms241612664Read this paper
Datasheet
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