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HMGB2 Recombinant Antibody

BSM-60675R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHMGB2
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Overview

  • Supplier
    Bioss
  • Product Name
    HMGB2 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-1000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:50-100), IF(IF=1:50), ICC/IF(ICC/IF=1:50)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3148
  • Target name
    HMGB2
  • Target description
    high mobility group box 2
  • Target synonyms
    HMG2, high mobility group protein B2, HMG-2, high mobility group protein 2, high-mobility group (nonhistone chromosomal) protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26583
  • Protein Name
    High mobility group protein B2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HMGB2 using anti-HMGB2 antibody (PB10002). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGB2 antigen affinity purified polyclonal antibody (Catalog # PB10002) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGB2 at approximately 24 kDa. The expected band size for HMGB2 is at 24 kDa.
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