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Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA010570ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA010570ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA010570ESR2HU at dilution of 1:100

HMGN2 Antibody

CSB-PA010570ESR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHMGN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    HMGN2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3151
  • Target name
    HMGN2
  • Target description
    high mobility group nucleosomal binding domain 2
  • Target synonyms
    HMG17, non-histone chromosomal protein HMG-17, high mobility group nucleosome-binding domain-containing protein 2, high mobility group protein N2, high-mobility group (nonhistone chromosomal) protein 17, nonhistone chromosomal protein HMG-17
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05204
  • Protein Name
    Non-histone chromosomal protein HMG-17
  • Scientific Description
    Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HMGN2 using anti-HMGN2 antibody (A04839-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGN2 antigen affinity purified polyclonal antibody (Catalog # A04839-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGN2 at approximately 18 kDa. The expected band size for HMGN2 is at 18 kDa.
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