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ProductsBack/HNF6 Polyclonal Antibody

HNF6 Polyclonal Antibody

BS-18062R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rat, Sheep
TargetONECUT1
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Overview

  • Supplier
    Bioss
  • Product Name
    HNF6 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3175
  • Target name
    ONECUT1
  • Target description
    one cut homeobox 1
  • Target synonyms
    HNF-6, HNF6, HNF6A, hepatocyte nuclear factor 6, hepatocyte nuclear factor 6, alpha, one cut domain family member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBC0
  • Protein Name
    Hepatocyte nuclear factor 6
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of ONECUT1 using anti-ONECUT1 antibody (A07349). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT1 antigen affinity purified polyclonal antibody (Catalog # A07349) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT1 at approximately 50 kDa. The expected band size for ONECUT1 is at 51,51-55 kDa.
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