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Western Blot Positive WB detected in: Jurkat whole cell lysate, Rat brain tissue All lanes: ONECUT1 antibody at 7.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate, Rat brain tissue All lanes: ONECUT1 antibody at 7.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate, Rat brain tissue All lanes: ONECUT1 antibody at 7.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa

ONECUT1 Antibody

CSB-PA880058LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetONECUT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ONECUT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3175
  • Target name
    ONECUT1
  • Target description
    one cut homeobox 1
  • Target synonyms
    hepatocyte nuclear factor 6; hepatocyte nuclear factor 6, alpha; HNF6; HNF-6; HNF6A; one cut domain family member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBC0
  • Protein Name
    Hepatocyte nuclear factor 6
  • Scientific Description
    Transcriptional activator. Binds the consensus sequence 5-DHWATTGAYTWWD-3 on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ONECUT1 using anti-ONECUT1 antibody (A07349). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT1 antigen affinity purified polyclonal antibody (Catalog # A07349) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT1 at approximately 50 kDa. The expected band size for ONECUT1 is at 51,51-55 kDa.
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Immunohistochemistry analysis in human liver and small intestine tissues using Anti-ONECUT1 antibody. Corresponding ONECUT1 RNA-seq data are presented for the same tissues.
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