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ProductsBack/HNLF / TMED4 Antibody (aa134-183, Biotin)

HNLF / TMED4 Antibody (aa134-183, Biotin)

LS-C441759
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Equine, Guinea Pig, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Xenopus, Zebra Fish
TargetTMED4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HNLF / TMED4 Antibody (aa134-183, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID222068
  • Target name
    TMED4
  • Target description
    transmembrane p24 trafficking protein 4
  • Target synonyms
    ERS25, GMP25iso, HNLF, p24a3, p24alpha3, transmembrane emp24 domain-containing protein 4, endoplasmic reticulum stress-response protein 25 kDa, p24 family protein alpha-3, putative NF-kappa-B-activating protein 156, putative NFkB activating protein HNLF, transmembrane emp24 protein transport domain containing 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Equine, Guinea Pig, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Xenopus, Zebra Fish
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMED4 using anti-TMED4 antibody (A12647-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED4 antigen affinity purified polyclonal antibody (Catalog # A12647-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED4 at approximately 25 kDa. The expected band size for TMED4 is at 26 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA639635(TMED4 Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: x200)
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Lane 1: Mouse Kidney lysates probed with TMED4 Polyclonal Antibody, Unconjugated (bs-6208R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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