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ProductsBack/hnRNP F (HNRNPF) Mouse Monoclonal Antibody [Clone ID: OTI5F5]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and HNRNPF-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF monoclonal antibody TA500804(1:5000). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and HNRNPF-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF monoclonal antibody TA500804(1:5000). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and HNRNPF-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF monoclonal antibody TA500804(1:5000). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

hnRNP F (HNRNPF) Mouse Monoclonal Antibody [Clone ID: OTI5F5]

CF500804
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHNRNPF
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    hnRNP F (HNRNPF) Mouse Monoclonal Antibody [Clone ID: OTI5F5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5F5
  • Gene ID3185
  • Target name
    HNRNPF
  • Target description
    heterogeneous nuclear ribonucleoprotein F
  • Target synonyms
    HNRPF, OK/SW-cl.23, mcs94-1, heterogeneous nuclear ribonucleoprotein F, HnRNP F protein, nucleolin-like protein mcs94-1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP52597
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein F
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HNRNPF mouse monoclonal antibody, clone OTI5F5 (formerly 5F5)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HnRNPF using anti-HnRNPF antibody (A05806). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SKOV3 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human U20S whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HnRNPF antigen affinity purified polyclonal antibody (Catalog # A05806) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HnRNPF at approximately 46 kDa. The expected band size for HnRNPF is at 46 kDa.
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