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Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue, Mouse brain tissue, Mouse kidney tissue All lanes: HNRNPL antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 51 kDa Observed band size: 65 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue, Mouse brain tissue, Mouse kidney tissue All lanes: HNRNPL antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 51 kDa Observed band size: 65 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue, Mouse brain tissue, Mouse kidney tissue All lanes: HNRNPL antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 51 kDa Observed band size: 65 kDa

HNRNPL Antibody

CSB-PA010612LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHNRNPL
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNRNPL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3191
  • Target name
    HNRNPL
  • Target description
    heterogeneous nuclear ribonucleoprotein L
  • Target synonyms
    HNRPL, P/OKcl.14, hnRNP-L, heterogeneous nuclear ribonucleoprotein L, epididymis secretory sperm binding protein, hnRNP L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14866
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein L
  • Scientific Description
    Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP L/HNRNPL antigen affinity purified polyclonal antibody (Catalog # A03769-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP L/HNRNPL at approximately 68 kDa. The expected band size for hnRNP L/HNRNPL is at 64 kDa.
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ReactivityHuman, Mouse, Rat
TargetHNRNPL
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