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HNRPD Antibody Pair

CSB-EAP02544
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Sheep
TargetHNRNPD
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNRPD Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.5ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID3184
  • Target name
    HNRNPD
  • Target description
    heterogeneous nuclear ribonucleoprotein D
  • Target synonyms
    AUF1, AUF1A, HNRPD, P37, hnRNPD0, heterogeneous nuclear ribonucleoprotein D0, ARE-binding protein AUFI, type A, AU-rich RNA-binding factor 1, AU-rich element RNA binding protein 1, 37kDa, hnRNP D0
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14103
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein D0
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of HNRNPD using anti-HNRNPD antibody (A09982-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPD antigen affinity purified polyclonal antibody (Catalog # A09982-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for HNRNPD at approximately 37-45KD. The expected band sizes for HNRNPD are at 37, 40, 42 and 45KD.
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