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HOMER1 / Homer 1 Antibody

LS-C814867
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetHOMER1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HOMER1 / Homer 1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9456
  • Target name
    HOMER1
  • Target description
    homer scaffold protein 1
  • Target synonyms
    HOMER, HOMER1A, HOMER1B, HOMER1C, SYN47, Ves-1, homer protein homolog 1, homer homolog 1, homer scaffolding protein 1, homer, neuronal immediate early gene, 1, homer-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HOMER1 using anti-HOMER1 antibody (A03877-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOMER1 antigen affinity purified polyclonal antibody (Catalog # A03877-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOMER1 at approximately 50 kDa. The expected band size for HOMER1 is at 50 kDa.
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