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Western blot All lanes: HOOK2 antibody at 3.91microg/ml Lane 1: Mouse large intestine tissue Lane 2: Mouse stomach tissue Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa
Western blot All lanes: HOOK2 antibody at 3.91microg/ml Lane 1: Mouse large intestine tissue Lane 2: Mouse stomach tissue Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa
Western blot All lanes: HOOK2 antibody at 3.91microg/ml Lane 1: Mouse large intestine tissue Lane 2: Mouse stomach tissue Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa

HOOK2 Antibody

CSB-PA839313ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHOOK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    HOOK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29911
  • Target name
    HOOK2
  • Target description
    hook microtubule tethering protein 2
  • Target synonyms
    HK2, protein Hook homolog 2, h-hook2, hHK2, hook homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96ED9
  • Protein Name
    Protein Hook homolog 2
  • Scientific Description
    Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HOOK2 using anti-HOOK2 antibody (A08854-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOOK2 antigen affinity purified polyclonal antibody (Catalog # A08854-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOOK2 at approximately 83 kDa. The expected band size for HOOK2 is at 83 kDa.
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