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HOOK2 Antibody

LS-C834948
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHOOK2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HOOK2 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:30 - 1:150)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29911
  • Target name
    HOOK2
  • Target description
    hook microtubule tethering protein 2
  • Target synonyms
    HK2, protein Hook homolog 2, h-hook2, hHK2, hook homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: HOOK2 antibody at 3.91microg/ml Lane 1: Mouse large intestine tissue Lane 2: Mouse stomach tissue Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa
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Figure 1. Western blot analysis of HOOK2 using anti-HOOK2 antibody (A08854-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOOK2 antigen affinity purified polyclonal antibody (Catalog # A08854-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOOK2 at approximately 83 kDa. The expected band size for HOOK2 is at 83 kDa.
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