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Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Rat heart tissue All lanes: HOXA5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 49 kDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Rat heart tissue All lanes: HOXA5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 49 kDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Rat heart tissue All lanes: HOXA5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 49 kDa

HOXA5 Antibody

CSB-PA010655LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetHOXA5
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Overview

  • Supplier
    Cusabio
  • Product Name
    HOXA5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3202
  • Target name
    HOXA5
  • Target description
    homeobox A5
  • Target synonyms
    HOX1, HOX1.3, HOX1C, homeobox protein Hox-A5, homeo box 1C, homeo box A5, homeobox protein HOXA5, homeobox protein Hox-1C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20719
  • Protein Name
    Homeobox protein Hox-A5
  • Scientific Description
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5-CYYNATTA[TG]Y-3.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HOXA5 using anti-HOXA5 antibody (A04018-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA5 antigen affinity purified polyclonal antibody (Catalog # A04018-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA5 at approximately 29KD. The expected band size for HOXA5 is at 29KD.
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