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Western Blot analysis of Hela cells using Hox-B9 Polyclonal Antibody
Western Blot analysis of Hela cells using Hox-B9 Polyclonal Antibody
Western Blot analysis of Hela cells using Hox-B9 Polyclonal Antibody

HOXB9 Antibody

CSB-PA002963
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHOXB9
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Overview

  • Supplier
    Cusabio
  • Product Name
    HOXB9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3219
  • Target name
    HOXB9
  • Target description
    homeobox B9
  • Target synonyms
    homeo box 2E; homeo box B9; homeobox protein Hox-2.5; homeobox protein Hox-2E; homeobox protein Hox-B9; HOX2; HOX-2.5; HOX2E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17482
  • Protein Name
    Homeobox protein Hox-B9
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HOXB9 using anti-HOXB9 antibody (A05689-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXB9 antigen affinity purified polyclonal antibody (Catalog # A05689-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXB9 at approximately 32KD. The expected band size for HOXB9 is at 32KD.
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