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WB analysis of K562 cell lysates using GTX87588 HOXB9 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of K562 cell lysates using GTX87588 HOXB9 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of K562 cell lysates using GTX87588 HOXB9 antibody. The lane on the right is blocked with the synthesized peptide.

HOXB9 antibody

GTX87588
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetHOXB9
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Overview

  • Supplier
    GeneTex
  • Product Name
    HOXB9 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3219
  • Target name
    HOXB9
  • Target description
    homeobox B9
  • Target synonyms
    homeo box 2E; homeo box B9; homeobox protein Hox-2.5; homeobox protein Hox-2E; homeobox protein Hox-B9; HOX2; HOX-2.5; HOX2E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17482
  • Protein Name
    Homeobox protein Hox-B9
  • Scientific Description
    This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded nuclear protein functions as a sequence-specific transcription factor that is involved in cell proliferation and differentiation. Increased expression of this gene is associated with some cases of leukemia, prostate cancer and lung cancer. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HOXB9 using anti-HOXB9 antibody (A05689-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXB9 antigen affinity purified polyclonal antibody (Catalog # A05689-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXB9 at approximately 32KD. The expected band size for HOXB9 is at 32KD.
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