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The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA091932(HOXC8 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA091932(HOXC8 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA091932(HOXC8 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

HOXC8 Antibody

CSB-PA091932
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHOXC8
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Overview

  • Supplier
    Cusabio
  • Product Name
    HOXC8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3224
  • Target name
    HOXC8
  • Target description
    homeobox C8
  • Target synonyms
    HOX3, HOX3A, homeobox protein Hox-C8, Hox-3.1, mouse, homolog of, homeo box 3A, homeo box C8, homeobox protein Hox-3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31273
  • Protein Name
    Homeobox protein Hox-C8
  • Scientific Description
    This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The product of this gene may play a role in the regulation of cartilage differentiation. It could also be involved in chondrodysplasias or other cartilage disorders.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HOXC8 using anti-HOXC8 antibody (A06655-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXC8 antigen affinity purified polyclonal antibody (Catalog # A06655-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXC8 at approximately 35KD. The expected band size for HOXC8 is at 35KD.
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Paraformaldehyde-fixed, paraffin embedded Human Cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HOXc8 Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, DAB staining.
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