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Paraformaldehyde-fixed, paraffin embedded Human Cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HOXc8 Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HOXc8 Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HOXc8 Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, DAB staining.

HOXc8 Polyclonal Antibody

BS-0394R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHOXC8
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Overview

  • Supplier
    Bioss
  • Product Name
    HOXc8 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3224
  • Target name
    HOXC8
  • Target description
    homeobox C8
  • Target synonyms
    HOX3, HOX3A, homeobox protein Hox-C8, Hox-3.1, mouse, homolog of, homeo box 3A, homeo box C8, homeobox protein Hox-3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HOXC8 using anti-HOXC8 antibody (A06655-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXC8 antigen affinity purified polyclonal antibody (Catalog # A06655-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXC8 at approximately 35KD. The expected band size for HOXC8 is at 35KD.
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The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA091932(HOXC8 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
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