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HP1 gamma antibody

GTX117561
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCBX3
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Overview

  • Supplier
    GeneTex
  • Product Name
    HP1 gamma antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11335
  • Target name
    CBX3
  • Target description
    chromobox 3
  • Target synonyms
    chromobox homolog 3 (HP1 gamma homolog, Drosophila); chromobox protein homolog 3; HECH; heterochromatin protein 1 homolog gamma; heterochromatin protein HP1 gamma; heterochromatin-like protein 1; HP1 gamma homolog; HP1-GAMMA; HP1Hs-gamma; modifier 2 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13185
  • Protein Name
    Chromobox protein homolog 3
  • Scientific Description
    At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5 UTR, have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Histone H1 acetylation at lysine 85 regulates chromatin condensation and genome stability upon DNA damage. Li Y et al., 2018 Sep 6, Nucleic Acids Res
    Read more

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Figure 1. Western blot analysis of HP1 gamma using anti-HP1 gamma antibody (A01142). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysate, Lane 3: rat testis tissue lysate, Lane 4: rat PC-12 whole cell lysate, Lane 5: mouse brain tissue lysate, Lane 6: mouse brain tissue lysate, Lane 7: mouse testis tissue lysate, Lane 8: mouse HEPA1-6 whole cell lysate, Lane 9: mouse NIH/3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP1 gamma antigen affinity purified polyclonal antibody (Catalog # A01142) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP1 gamma at approximately 21KD. The expected band size for HP1 gamma is at 21KD.
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