Wild-type (WT) and HPRT1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637916/GTX637916_T-44872_20221223_WB_M_testis_22122722_321.webp "Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637916/GTX637916_T-44872_20221223_WB_R_testis_22122722_616.webp "Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637916/GTX637916_T-44872_20221230_IHC-P_multiple_M_23020621_871.webp "HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637916/GTX637916_44928_20230203_WB_TPM_watermark_23021401_289.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637916/GTX637916_T-44872_20230519_ICC_IF_R_23053001_177.webp "HPRT antibody [HL2015] detects HPRT protein at cell membrane by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).")
![Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637916/GTX637916_44928_20230616_WB_D_23062019_336.webp "Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637916/GTX637916_44928_20230616_WB_Z_23062019_630.webp "Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HPRT antibody [HL2015] detects HPRT protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat intestine. HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637916/GTX637916_44928_20230714_IHC-P_Cat_23081619_996.webp "HPRT antibody [HL2015] detects HPRT protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat intestine. HPRT stained by HPRT antibody [HL2015] (GTX637916) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Whole Japanese medaka extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637916/GTX637916_44928_20250815_WB_medaka_25082121_340.webp "Whole Japanese medaka extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Wild-type (WT) and HPRT1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody [HL2015] (GTX637916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HPRT antibody [HL2015]
GTX637916
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
TargetHPRT1
Overview
- SupplierGeneTex
- Product NameHPRT antibody [HL2015]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2015
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3251
- Target nameHPRT1
- Target descriptionhypoxanthine phosphoribosyltransferase 1
- Target synonymsHGPRT, HPRT, hypoxanthine-guanine phosphoribosyltransferase, HGPRTase, hypoxanthine guanine phosphoribosyl transferase, hypoxanthine-guanine phosphoribosyltransferase 1, testicular tissue protein Li 89
- HostRabbit
- IsotypeIgG
- Protein IDP00492
- Protein NameHypoxanthine-guanine phosphoribosyltransferase
- Scientific DescriptionThe protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]
- ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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