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Western Blot analysis of COLO205 cells using Histamine H3 Receptor Polyclonal Antibody
Western Blot analysis of COLO205 cells using Histamine H3 Receptor Polyclonal Antibody
Western Blot analysis of COLO205 cells using Histamine H3 Receptor Polyclonal Antibody

HRH3 Antibody

CSB-PA006801
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHRH3
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Overview

  • Supplier
    Cusabio
  • Product Name
    HRH3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11255
  • Target name
    HRH3
  • Target description
    histamine receptor H3
  • Target synonyms
    G protein-coupled receptor 97; GPCR97; H3R; HH3R; histamine H3 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5N1
  • Protein Name
    Histamine H3 receptor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HRH3 using anti-HRH3 antibody (M06436). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRH3 antigen affinity purified monoclonal antibody (M06436) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRH3 at approximately 45 kDa. The expected band size for HRH3 is at 49 kDa.
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