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Western Blot Positive WB detected in: Rat spleen tissue All lanes: HS2ST1 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 35, 27 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: Rat spleen tissue All lanes: HS2ST1 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 35, 27 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: Rat spleen tissue All lanes: HS2ST1 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 35, 27 kDa Observed band size: 42 kDa

HS2ST1 Antibody

CSB-PA010746LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetHS2ST1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HS2ST1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9653
  • Target name
    HS2ST1
  • Target description
    heparan sulfate 2-O-sulfotransferase 1
  • Target synonyms
    2-OST, NFSRA, dJ604K5.2, heparan sulfate 2-O-sulfotransferase 1, 2-O-sulfotransferase, 2OST, HS 2-O-sulfotransferase, heparan sulfate 2-sulfotransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7LGA3
  • Protein Name
    Heparan sulfate 2-O-sulfotransferase 1
  • Scientific Description
    Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high-affinity ligand-binding interactions and signaling processes. Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues (By similarity).
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HS2ST1 using anti-HS2ST1 antibody (A11487). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HS2ST1 antigen affinity purified polyclonal antibody (Catalog # A11487) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HS2ST1 at approximately 42 kDa. The expected band size for HS2ST1 is at 42 kDa.
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