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Lane 1: HepG2 cell lysates; Lane 2: Mouse lung lysates probed with HS2ST1 Polyclonal Antibody, Unconjugated (bs-17389R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: Mouse lung lysates probed with HS2ST1 Polyclonal Antibody, Unconjugated (bs-17389R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: Mouse lung lysates probed with HS2ST1 Polyclonal Antibody, Unconjugated (bs-17389R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

HS2ST1 Polyclonal Antibody

BS-17389R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetHS2ST1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HS2ST1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9653
  • Target name
    HS2ST1
  • Target description
    heparan sulfate 2-O-sulfotransferase 1
  • Target synonyms
    2OST; 2-O-sulfotransferase; dJ604K5.2; heparan sulfate 2-O-sulfotransferase 1; NFSRA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7LGA3
  • Protein Name
    Heparan sulfate 2-O-sulfotransferase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HS2ST1 using anti-HS2ST1 antibody (A11487). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HS2ST1 antigen affinity purified polyclonal antibody (Catalog # A11487) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HS2ST1 at approximately 42 kDa. The expected band size for HS2ST1 is at 42 kDa.
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