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Western Blot Positive WB detected in: 293 whole cell lysate All lanes: HSD11B2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: 293 whole cell lysate All lanes: HSD11B2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: 293 whole cell lysate All lanes: HSD11B2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa

HSD11B2 Antibody

CSB-PA010765LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHSD11B2
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSD11B2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3291
  • Target name
    HSD11B2
  • Target description
    hydroxysteroid 11-beta dehydrogenase 2
  • Target synonyms
    AME, AME1, HSD11K, HSD2, SDR9C3, 11-beta-hydroxysteroid dehydrogenase type 2, -HSD11 type II, 11-DH2, 11-HSD type II, 11-beta-HSD type II, 11-beta-HSD2, 11-beta-hydroxysteroid dehydrogenase type II, NAD-dependent 11-beta-hydroxysteroid dehydrogenase, corticosteroid 11-beta-dehydrogenase isozyme 2, short chain dehydrogenase/reductase family 9C member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP80365
  • Protein Name
    Corticosteroid 11-beta-dehydrogenase isozyme 2
  • Scientific Description
    Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B2 antigen affinity purified polyclonal antibody (Catalog # A01613) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B2 at approximately 40 kDa. The expected band size for HSD11B2 is at 44 kDa.
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