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Mouse kidney lysates probed with HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse kidney lysates probed with HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse kidney lysates probed with HSD11B2 Polyclonal Antibody, Unconjugated (bs-3618R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

HSD11B2 Polyclonal Antibody

BS-3618R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHSD11B2
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Overview

  • Supplier
    Bioss
  • Product Name
    HSD11B2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3291
  • Target name
    HSD11B2
  • Target description
    hydroxysteroid 11-beta dehydrogenase 2
  • Target synonyms
    AME, AME1, HSD11K, HSD2, SDR9C3, 11-beta-hydroxysteroid dehydrogenase type 2, -HSD11 type II, 11-DH2, 11-HSD type II, 11-beta-HSD type II, 11-beta-HSD2, 11-beta-hydroxysteroid dehydrogenase type II, NAD-dependent 11-beta-hydroxysteroid dehydrogenase, corticosteroid 11-beta-dehydrogenase isozyme 2, short chain dehydrogenase/reductase family 9C member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP80365
  • Protein Name
    11-beta-hydroxysteroid dehydrogenase type 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang F, Zhang Q, Zhang X, et al. Preeclampsia induced by cadmium in rats is related to abnormal local glucocorticoid synthesis in placenta. Reprod Biol Endocrinol. 2014,12:77. doi: 10.1186/1477-7827-12-77
    Read this paper
  • Xu Z, Zhao F, Lin F, et al. Preeclampsia is associated with a deficiency of lipoxin A4, an endogenous anti-inflammatory mediator. Fertil Steril. 2014,102(1):282-290.e4. doi: 10.1016/j.fertnstert.2014.03.056
    Read this paper

Datasheet

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Figure 1. Western blot analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B2 antigen affinity purified polyclonal antibody (Catalog # A01613) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B2 at approximately 40 kDa. The expected band size for HSD11B2 is at 44 kDa.
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