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Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-HSD17B1 Polyclonal Antibody, Unconjugated (bs-3855R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-HSD17B1 Polyclonal Antibody, Unconjugated (bs-3855R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-HSD17B1 Polyclonal Antibody, Unconjugated (bs-3855R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

HSD17B1 Polyclonal Antibody

BS-3855R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetHSD17B1
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Overview

  • Supplier
    Bioss
  • Product Name
    HSD17B1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3292
  • Target name
    HSD17B1
  • Target description
    hydroxysteroid 17-beta dehydrogenase 1
  • Target synonyms
    17-beta-HSD, 20-alpha-HSD, E2DH, EDH17B2, EDHB17, HSD17, SDR28C1, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, estradiol 17-beta-dehydrogenase 1, placental 17-beta-hydroxysteroid dehydrogenase, short chain dehydrogenase/reductase family 28C member 1, short chain dehydrogenase/reductase family 28CE, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14061
  • Protein Name
    17-beta-hydroxysteroid dehydrogenase type 1
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhang W, Zhao Y, Zhang P, et al. Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Chemosphere. 2018,194:147-157. doi: 10.1016/j.chemosphere.2017.11.164
    Read this paper

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Figure 1. Western blot analysis of HSD17B1 using anti-HSD17B1 antibody (A02198). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Human Placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B1 antigen affinity purified polyclonal antibody (Catalog # A02198) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B1 at approximately 40KD. The expected band size for HSD17B1 is at 35KD.
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