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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA581084(HSD17B13 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA581084(HSD17B13 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA581084(HSD17B13 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)

HSD17B13 Antibody

CSB-PA581084
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetHSD17B13
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSD17B13 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID345275
  • Target name
    HSD17B13
  • Target description
    hydroxysteroid 17-beta dehydrogenase 13
  • Target synonyms
    17-beta hydroxysteroid dehydrogenase; 17-beta-HSD 13; 17-beta-hydroxysteroid dehydrogenase 13; HMFN0376; NIIL497; SCDR9; SDR16C3; short chain dehydrogenase/reductase family 16C member 3; short-chain dehydrogenase/reductase 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z5P4
  • Protein Name
    17-beta-hydroxysteroid dehydrogenase 13
  • Scientific Description
    17beta-HSD13 (17beta hydroxysteroid dehydrogenase type 13), also designated Short-chain dehydrogenase/reductase 9 (SCDR9), belongs to the 17beta-HSD family of proteins, which regulate the availability of steroids within various tissues throughout the body. 17beta-HSD13 is a 300 amino acid secreted protein that is highly expressed in liver and is also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis. The gene encoding 17beta-HSD13 maps to chromosome 4, which houses nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSD17B13 using anti-HSD17B13 antibody (A12700-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 2: rat liver tissue lysates, Lane 3: rat RH-35 whole cell lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B13 antigen affinity purified polyclonal antibody (Catalog # A12700-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B13 at approximately 34 kDa. The expected band size for HSD17B13 is at 34 kDa.
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