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WB analysis of various sample lysates using GTX33250 HSD17B13 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33250 HSD17B13 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33250 HSD17B13 antibody. Dilution : 1:1000 Loading : 25microg per lane

HSD17B13 antibody

GTX33250
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHSD17B13
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Overview

  • Supplier
    GeneTex
  • Product Name
    HSD17B13 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID345275
  • Target name
    HSD17B13
  • Target description
    hydroxysteroid 17-beta dehydrogenase 13
  • Target synonyms
    FLDP, HMFN0376, NIIL497, SCDR9, SDR16C3, 17-beta-hydroxysteroid dehydrogenase 13, 17-beta hydroxysteroid dehydrogenase, 17-beta-HSD 13, hepatic retinol/retinal dehydrogenase, short chain dehydrogenase/reductase family 16C member 3, short-chain dehydrogenase/reductase 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z5P4
  • Protein Name
    17-beta-hydroxysteroid dehydrogenase 13
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of HSD17B13 using anti-HSD17B13 antibody (A12700-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 2: rat liver tissue lysates, Lane 3: rat RH-35 whole cell lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B13 antigen affinity purified polyclonal antibody (Catalog # A12700-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B13 at approximately 34 kDa. The expected band size for HSD17B13 is at 34 kDa.
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