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HSD17B2 Polyclonal Antibody

RD82453A
Reddot Biotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSD17B2

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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    HSD17B2 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3294
  • Target name
    HSD17B2
  • Target description
    hydroxysteroid 17-beta dehydrogenase 2
  • Target synonyms
    EDH17B2, HSD17, SDR9C2, 17-beta-hydroxysteroid dehydrogenase type 2, 17-beta-HSD 2, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH, estradiol 17-beta-dehydrogenase 2, microsomal 17-beta-hydroxysteroid dehydrogenase, short chain dehydrogenase/reductase family 9C member 2, testosterone 17-beta-dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    17beta-HSD2 (17beta hydroxysteroid dehydrogenase type 2) belongs to the 17beta-HSD family of proteins that regulate the availability of steroids within a tissue. 17beta-HSD2 converts active steroids to their inactive form through its oxidative activity. It is a key player in the inactivation of Estradiol and testosterone. Due to the affects that 17beta-HSD2 has on the availability of estrogen, it has been extensively investigated for playing a possible role in breast tumor development, colon cancer development and the pathophysiology of endometriosis.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HSD17B2 using anti-HSD17B2 antibody (A02506-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: mouse liver tissue tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B2 antigen affinity purified polyclonal antibody (Catalog # A02506-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B2 at approximately 37 kDa. The expected band size for HSD17B2 is at 43-49,35-40 kDa.
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