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HSD17B7 Antibody, Biotin conjugated

CSB-PA010776LD01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetHSD17B7
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSD17B7 Antibody, Biotin conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID51478
  • Target name
    HSD17B7
  • Target description
    hydroxysteroid 17-beta dehydrogenase 7
  • Target synonyms
    PRAP, SDR37C1, 3-keto-steroid reductase/17-beta-hydroxysteroid dehydrogenase 7, 17 beta-hydroxysteroid dehydrogenase type VII, 17-beta-HSD 7, 17-beta-hydroxysteroid dehydrogenase 7, 17beta hydroxysteroid dehydrogenase, 3-keto-steroid reductase, dihydrotestosterone oxidoreductase, estradiol 17-beta-dehydrogenase 7, short chain dehydrogenase/reductase family 37C member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56937
  • Protein Name
    3-keto-steroid reductase/17-beta-hydroxysteroid dehydrogenase 7
  • Scientific Description
    Responsible for the reduction of the keto group on the C-3 of sterols.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSD17B7 using anti-HSD17B7 antibody (A05850-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B7 antigen affinity purified polyclonal antibody (Catalog # A05850-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B7 at approximately 38 kDa. The expected band size for HSD17B7 is at 38 kDa.
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