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ProductsBack/HSF1 Rat Monoclonal Antibody [Clone ID: 4B4]

HSF1 Rat Monoclonal Antibody [Clone ID: 4B4]

AM00238PU-N
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSF1
Price on request
100 ug
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More than 130 suppliers
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Overview

  • Supplier
    OriGene
  • Product Name
    HSF1 Rat Monoclonal Antibody [Clone ID: 4B4]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4B4
  • Gene ID3297
  • Target name
    HSF1
  • Target description
    heat shock transcription factor 1
  • Target synonyms
    HSTF1, heat shock factor protein 1
  • Host
    Rat
  • Isotype
    IgG1
  • Protein IDQ00613
  • Protein Name
    Heat shock factor protein 1
  • Scientific Description
    HSF1 rat monoclonal antibody, clone 4B4, Purified
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HSF1 using anti-HSF1 antibody (A00250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF1 antigen affinity purified polyclonal antibody (Catalog # A00250) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF1 at approximately 80 kDa. The expected band size for HSF1 is at 57 kDa.
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