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IHC-P analysis of human breast carcinoma tissue using GTX87716 Hsp20 antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human breast carcinoma tissue using GTX87716 Hsp20 antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human breast carcinoma tissue using GTX87716 Hsp20 antibody. The picture on the right is blocked with the synthesized peptide.

Hsp20 antibody

GTX87716
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetHSPB6
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Overview

  • Supplier
    GeneTex
  • Product Name
    Hsp20 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID126393
  • Target name
    HSPB6
  • Target description
    heat shock protein family B (small) member 6
  • Target synonyms
    epididymis luminal protein 55; epididymis secretory sperm binding protein; heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein family B (small) member B6; heat shock protein, alpha-crystallin-related, B6; HEL55; Hsp20; PPP1R91; protein phosphatase 1, regulatory subunit 91
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14558
  • Protein Name
    Heat shock protein beta-6
  • Scientific Description
    This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation. [provided by RefSeq, Jan 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hsp20 using anti-Hsp20 antibody (A07981-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysate,Lane 2: rat skeletal muscle tissue lysate,Lane 3: rat testis tissue lysate,Lane 4: mouse testis tissue lysate,Lane 5: human A431 whole Cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp20 antigen affinity purified polyclonal antibody (Catalog # A07981-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp20 at approximately 17KD. The expected band size for Hsp20 is at 17KD.
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